Lowicryl Resin Kits for low temperature embedding
These embedding resins are based on a highly cross-linked acrylate and methacrylate formula developed by Carlemalm et al in Professor Kellenberger's laboratory.
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These Lowicryl® embedding resins are based on a highly cross-linked acrylate and methacrylate formula developed by Carlemalm et al in Professor Kellenberger's laboratory. They have been designed for use over a wide range of embedding conditions and have been formulated to provide low viscosity at low temperature; K4M down to -35°C and HM20 to -70°C. K4M is a water compatible polar embedding resin while HM20 is a hydrophobic non-polar resin. Both resins may be polymerised by ultraviolet light (360nm) at low temperatures or at room temperature. Thermal polymerisation is also possible. Dehydration, infiltration and embedding are carried out at low temperatures reducing the extraction of lipids. These resins permit adequate retention of ultrastructure, together with improved preservation of antigenicity.
K4M kit:
- K4M resin (monomer B). 3 x 250ml
- K4M cross-linker (A). 130ml
- K4M initiator (C). 5g
HM20 kit:
- HM20 resin (monomer E). 3 x 250ml
- HM20 cross-linker (D). 130ml
- HM20 initiator (C). 5g
Lowicryl K11M and HM23 kits have similar properties to K4M and HM20 but have been developed for use at significantly lower temperatures. They are based on acrylate and methacrylate esters with freezing points that allow applications down to approx -60°C and -80°C for K11M and HM23 respectively. K11M is hydrophilic and HM23 hydrophobic. Both resins have been specifically designed for freeze substitution, together with low temperature embedding. K11M has a lower viscosity than K4M.
K11M kit:
- K11M resin (monomer I). 3 x 250ml
- K11M cross-linker (H). 50ml
- K11M initiator (C). 5g
HM23 kit:
- HM23 resin (monomer G). 250ml
- HM23 cross-linker (F). 130ml
- HM23 initiator (C). 5g (for polymerisation above -50°C)
- HM23 initiator (J). 5g (for polymerisation -50°C and below
