Amorphous Nickel-Titanium Holey Foil Grids for Cryo-EM (ANTcryo™) features high porosity, excellent conductivity, and user-friendly advantages.
Key Advantages:
- Improves the resolution of reconstruction
- Improves the rate of sample preparation
- Improves the efficiency of data collection
ANTcryo grids are available in a variety of standard product formats as well as available as customised items.
Standard Products:
- MDL-M01: Gold support grid, Ni-Ti holey foil, 300 mesh, 1.2/1.3 Aperture Pitch
- MDL-M02: Copper support grid, Ni-Ti holey foil, 300 mesh, 1.2/1.3 Aperture Pitch
- MDL-M03: Gold support grid, Ni-Ti holey foil, 400 mesh, 1.2/1.3 Aperture Pitch
- MDL-M04: Gold support grid, Ni-Ti holey foil, 300 mesh, 2.0/1.0 Aperture Pitch
Customised Products:
- Grid Type: Gold or Copper
- Mesh Size: 200, 300 or 400
- Aperture Pitch: 0.8/1.8; 1.2/1.3; 1.0/1.0; 2.0/2.0; 2.0/1.0
Usage Instructions
Differentiate ANTcryo™ film sides:
ANTcryo™ grids contain one side in a gray-black color, and the other side in the color of the bare metal grid. The side with the film is the front side, facing downward into the packaging box (please observe the markings on the packaging box)
Plasma Cleaning:
Place the grid with the film side facing up into a plasma cleaner for surface hydrophilization treatment.
Recommended parameters are as follows:
Gatan Solarus Plasma Cleaner: 50W, H2/O2, 1min
PELCO easiGlow™ Glow Discharge Cleaning System:15mA 30-60s
Sample Concentration:
Different samples require varying concentrations.
For example, Apoferritin is suitable at a concentration of 1-1.5mg/ml.
Cryo Sample Preparation:
The grid is compatible with commonly used Cryo sample preparation instruments. Follow the standard procedures of the equipment for sample preparation.