UA-Zero: Trouble-free EM Staining
UA-Zero Safe to use, safe to handle EM Stain
UA-Zero® EM Stain is a patented solution developed as a substitute for Uranyl Acetate. UA-Zero does not contain any radioactive material and is safe to use, transport and dispose of. It can be used as a direct replacement for Uranyl Acetate with no changes to standard user protocols and delivers high contrast imaging.
- Uranyl Acetate substitute
- Non-radioactive, Uranium free
- No handling, storage or disposal restrictions
- Image contrast results as good as Uranyl Acetate, if not better
- No additional processing required
- Direct replacement for Uranyl Acetate without changing standard user protocols
- Independently tested and verified
UA-Zero is non-radioactive and Uranium free. Consequently, there are none of the handling, storage or disposal issues associated with Uranyl Acetate.
The ready-to-use lead-free solution can be used as a direct replacement for Uranyl Acetate without any changes to the standard user protocols, and no additional processing is required to enhance the image contrast. As shown in the images below, the image contrast results carried out at Bristol University are as good as Uranyl Acetate, if not better.
UA-Zero is easy to use; to stain the sample on a TEM grid, with a support film, only a few simple steps are required before drying.
Image Comparisons
Comparison TEM images of HeLa cells grown on coverslips with samples prepared using UA-Zero EM Stain and Uranyl acetate, courtesy of Prof. Dr. Paul Verkade and Dr. Chris Neal, Wolfson Bioimaging Facility, School of Medical Sciences, University of Bristol.
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| TEM images of sample prepared using UA-Zero EM Stain in 20% ethanol as a staining solution. | TEM images of sample prepared using 3% Uranyl acetate in water as a staining solution. | TEM images of sample prepared using 3% Uranyl acetate in 20% ethanol as a staining solution. |
Application Notes
In collaboration with Bristol University, we have produced several Application Notes detailing the protocols and processes used to prepare stained specimens using both UA-Zero and Uranyl Acetate. Each Application Note contains TEM images comparing the results from UA-Zero with Uranyl Acetate.
- Fixation, staining & processing of HeLa cell pellet from culture dish
- Fixation, staining & processing of HeLa cells grown on coverslips
- Fixation, staining & processing of kidney tissue
- Tobacco Mosaic Virus (TMV) Negative staining